Coding

Part:BBa_K5310003:Design

Designed by: Ilias Pilianidis   Group: iGEM24_thessaloniki   (2024-09-28)


Myelin Basic Protein domain (aa 83-99)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First, because this region is immunogenic, it can elicit strong autoimmune reactions in certain primary T cell cultures or animal models. Thus, adequate controls and ethical considerations are required in experimental design. The purity and sequence faithfulness of synthetic peptides must also be validated, as minor differences may alter immunological outcomes.


Source

This peptide sequence comes from the human myelin basic protein (MBP), a critical protein found in the myelin sheath that insulates nerve fibers in the brain and spinal cord. MBP is encoded by the MBP gene on chromosome 18 in humans. The amino acid sequence 83-99 lies within the central immunogenic portion of the protein, which can be targeted by autoreactive T-cells in autoimmune diseases.

References